Part:BBa_K1088016:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQbfb298438d09ea06-partinfo-00000000-QINU UNIQbfb298438d09ea06-partinfo-00000001-QINU

••• Patrick Rosendahl Andreassen

We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy.